14-3-3 sigma Antibody

Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, IF/ICC: 2-4 µg/mL, FACS: 1-3ug/10^6 cells, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P31947

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human partial protein corresponding to amino acids M1-S248 was used as the immunogen for the 14-3-3 sigma antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF/ICC, FACS, IHC-P, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the 14-3-3 sigma antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This 14-3-3 sigma antibody is available for research use only.

Storage Conditions

After reconstitution, the 14-3-3 sigma antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the 14-3-3 sigma antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) 22RV1, 5) rat lung, 6) rat PC-12, 7) mouse lung, 8) mouse HEPA1-6 and 9) mouse NIH3T3 lysate with 14-3-3 sigma antibody at 0.5ug/ml.