Ptpn22 Antibody

Protein tyrosine phosphatase, non-receptor type 22 (lymphoid), also known as PTPN22, is a protein that in humans is encoded by the PTPN22 gene. This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P29352

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant mouse partial protein corresponding to amino acids M1-Q309 was used as the immunogen for the Ptpn22 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Ptpn22 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Ptpn22 antibody is available for research use only.

Storage Conditions

After reconstitution, the Ptpn22 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Ptpn22 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear, cell membrane

Image Legend

IHC testing of FFPE mouse spleen tissue with Ptpn22 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.