XRCC1 Antibody

XRCC1 (X-ray Repair Complementing Defective in Chinese Hamster 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL, IF/ICC (FFPE) : 2-4 µg/mL

UniProt

P18887

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human partial protein corresponding to amino acids E538-A633 was used as the immunogen for the XRCC1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the XRCC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This XRCC1 antibody is available for research use only.

Storage Conditions

After reconstitution, the XRCC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the XRCC1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) HepG2, 5) A549, 6) SKOV3, 7) PANC-1 and 8) mouse testis and 9) rat testis lysate with XRCC1 antibody at 0.5ug/ml. Rountinely observed molecular weight: 69~90 kDa.