ETV6 Antibody / Tel

Transcription factor ETV6 is a protein that in humans is encoded by the ETV6 gene. This gene encodes an ETS family transcription factor. The product of this gene contains two functional domains: a N-terminal pointed (PNT) domain that is involved in protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain. Gene knockout studies in mice suggest that it is required for hematopoiesis and maintenance of the developing vascular network. This gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P41212

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human partial protein corresponding to amino acids E327-Q448 was used as the immunogen for the ETV6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the ETV6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ETV6 antibody is available for research use only.

Storage Conditions

After reconstitution, the ETV6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ETV6 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human 1) placenta, 2) HeLa, 3) 22RV1, 4) SKOV and 5) A549 lysate with ETV6 antibody at 0.5ug/ml. Predicted molecular weight ~53 kDa.