ICOS Antibody

Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9WVS0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant mouse partial protein corresponding to amino acids E21-S200 was used as the immunogen for the ICOS antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the ICOS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ICOS antibody is available for research use only.

Storage Conditions

After reconstitution, the ICOS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ICOS antibody should be determined by the researcher.

Location

Cytoplasmic, cell membrane

Image Legend

Western blot testing of 1) mouse stomach, 2) mouse spleen, 3) mouse thymus, 4) mouse small intestine, 5) rat thymus and 6) human placental lysate with ICOS antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa.