HP1 Antibody / HP1A [Biotin Conjugate]

Additional name (s) for this target protein: Heterochromatin protein 1 alpha, HP1 alpha; CBX5

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.3 µg/mL, Immunohistochemistry (FFPE) : suitable, ELISA (peptide) LOD: 1:128000

Host

Goat

Reactivity

Human

Immunogen

Amino acids NKRKSNFSNSADDIK were used as the immunogen for this HP1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA (peptide)

Purity

Antigen affinity

Format

Biotin Conjugate

Buffer

0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Reconstitution

Aliquot and store the HP1 antibody at -20oC.

Limitations

This HP1 antibody is available for research use only.

Storage Conditions

Aliquot and store the HP1 antibody at -20°C.

Formulation

0.5 mg/mL in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Applications Notes

Optimal dilution of the HP1 antibody should be determined by the researcher.1. Do not use a blocking agent or diluent that contains biotin (including milk and some BSA preparations) when testing with biotinylated antibodies. A non-animal protein (NAP) blocker is recommended.

Prediction Reactivity

Dog, Mouse

Image Legend

Western blot testing of HeLa lysate with biotinylated HP1 antibody at 0.1ug/ml. Predicted molecular weight ~22kDa. An NAP blocker was used in place of non-fat milk as blocking solution and diluent. (1)