Il6 Antibody

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, the IL6 gene is mapped to chromosome 7p15.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL

UniProt

P20607

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids KTIQLILKALEEFLKVTMRSTRQT from the rat protein were used as the immunogen for the Il6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Il6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Il6 antibody is available for research use only.

Storage Conditions

After reconstitution, the Il6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Il6 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) rat heart and 2) mouse heart tissue lysate with Il6 antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa but may be observed at higher molecular weights due to glycosylation.