MED9 Antibody / MED25

MED9 is a member of the mediator complex of proteins that promote activation of RNA polymerase II through direct interactions with transcription factors. This MED9 gene is located within the Smith-Magenis syndrome region on chromosome 17.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA (recombinant human protein) : 0.1-0.5 µg/mL (BSA-free format available)

UniProt

Q9NWA0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids A55-E146 was used as the immunogen for the MED9 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the MED9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MED9 antibody is available for research use only.

Storage Conditions

After reconstitution, the MED9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MED9 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of rat 1) brain, 2) heart, 3) kidney and moouse 4) brain, 5) heart, 6) kidney with MED9 antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.