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MED6 Antibody / ARC33

Mediator of RNA polymerase II transcription subunit 6 is one of the subunits of the Mediator complex. It is an enzyme that in humans is encoded by the MED6 gene. This family of proteins represent the transcriptional mediator protein subunit 6 that is required for activation of many RNA polymerase II promoters and which are conserved from yeast to humans.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, Direct ELISA (recombinant human protein) : 0.1-0.5 µg/mL (BSA-free format available)

UniProt

O75586

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids F40-Q246 was used as the immunogen for the MED6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the MED6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MED6 antibody is available for research use only.

Storage Conditions

After reconstitution, the MED6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MED6 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human A431, 2) human Jurkat, 3) human HepG2, 4) human PC-3, 5) human U-2 OS, 6) human A549, 7) human MCF7, 8) rat C6 and 9) mouse NIH 3T3 cell lysate with MED6 antibody. Predicted molecular weight ~28 kDa, commonly observed at 28-33 kDa.

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