HSP20 Antibody

Heat shock protein beta-6 is a protein that in humans is encoded by the HSPB6 gene. HSPB6, also known as hsp20, is a 17-kDa member of the small heat shock family of proteins. It is expressed in multiple tissues; however, HSPB6 is most highly and constitutively expressed in different types of muscle including vascular, airway, colonic, bladder, uterine smooth muscle, cardiac muscle and skeletal muscle. HSPB6 has specific functions for vasodilation, platelet function, and insulin resistance and in smooth and cardiac muscle.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL

UniProt

O14558

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids H73-L123 was used as the immunogen for the HSP20 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the HSP20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HSP20 antibody is available for research use only.

Storage Conditions

After reconstitution, the HSP20 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HSP20 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human A431 cells with HSP20 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.