Laminin gamma Antibody (G1-3)

Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an ?-chain, a ?-chain, and a ?-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL

UniProt

P02468, Q61092, Q9R0B6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acid sequences from Laminin gamma 1-3 (NKLNEIEGSLNKAKDEMKAS + DLEERVRRQRNHLHLLETSI + LQLDSHGALHHKLRQLEEES) were used as the immunogen for the Laminin gamma antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the Laminin gamma antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Laminin gamma antibody is available for research use only.

Storage Conditions

After reconstitution, the Laminin gamma antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Laminin gamma antibody should be determined by the researcher.

Location

Membrane, secreted (cytoplasmic in some tissue)

Image Legend

Western blot testing of 1) mouse lung, 2) rat heart, 3) rat lung and 4) human HeLa lysate with Laminin gamma antibody at 0.5ug/ml. Expected molecular weight: 172-200 kDa (G1 & G3), 130-140 kDa (G2) .