NFAT4 Antibody / NFATC3

Nuclear factor of activated T-cells, cytoplasmic 3 is a protein that in humans is encoded by the NFATC3 gene. The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells, Direct ELISA (recombinant human protein) : 0.1-0.5 µg/mL (BSA-free format available)

UniProt

Q12968

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids K630-L712 was used as the immunogen for the NFAT4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the NFAT4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NFAT4 antibody is available for research use only.

Storage Conditions

After reconstitution, the NFAT4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NFAT4 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 22RV1 cell lysate with NFAT4 antibody at 0.5ug/ml. Predicted molecular weight: ~116 kDa but can be observed at 140~150 kDa due to phosphorylation.