ATG3 Antibody / APG3

This ATG3 gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. And the protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL

UniProt

Q9NT62

Host

Rabbit

Reactivity

Human, Mouse, Monkey

Immunogen

A recombinant human protein corresponding to amino acids M1-T244 was used as the immunogen for the ATG3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the ATG3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ATG3 antibody is available for research use only.

Storage Conditions

After reconstitution, the ATG3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ATG3 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human K562, 2) human Raji, 3) monkey COS-7, 4) human HeLa and 5) mouse NIH 3T3 cell lysate with ATG3 antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.