Periostin Antibody

POSTN (Periostin), also known as PN or OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. It was found that purified recombinant PN supported adhesion of ovarian epithelial cells. And periostin was overexpressed by the majority of human primary breast cancers examined. Transfected tumor cell lines overexpressing periostin showed accelerated growth and angiogenesis as xenografts in immunocompromised animals. It was showed that extracellular periostin induced reentry of differentiated mammalian cardiomyocytes into the cell cycle.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA (recombinant rat protein) : 0.1-0.5 µg/mL (BSA-free format available)

UniProt

D3ZAF5

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

A recombinant rat protein corresponding to amino acids K577-Q810 was used as the immunogen for the Periostin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the Periostin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Periostin antibody is available for research use only.

Storage Conditions

After reconstitution, the Periostin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Periostin antibody should be determined by the researcher.

Location

Cytoplasm, extracellular space

Image Legend

Western blot testing of 1) rat stomach, 2) rat lung, 3) rat NRK cells, 4) mouse heart, 5) mouse intestine and 6) mouse lung lysate with Periostin antibody at 0.5ug/ml. Predicted molecular weight ~93 kDa.