VEGFR3 Antibody / FLT4

Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence/Immunocytochemistry (FFPE) : 2-4 µg/mL, Flow cytometry: 1-2ug/10^6 cells

UniProt

P35916

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids SCRGQHPLEWAWPGAQEAPATGDKDSEDT were used as the immunogen for the VEGFR3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the VEGFR3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This VEGFR3 antibody is available for research use only.

Storage Conditions

After reconstitution, the VEGFR3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the VEGFR3 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear, cell membrane

Image Legend

Western blot testing of human 1) HeLa, 2) MCF7 and 3) HepG2 cell lysate with VEGFR3 antibody at 0.5ug/ml. Predicted molecular weight ~153 kDa (long), ~147 kDa (short) and ~93 kDa (sVegfr3) .