PDCD4 Antibody

Programmed cell death protein 4 (PDCD4) is a protein that in humans is encoded by the PDCD4 gene. It is mapped to 10q25.2. This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. It also encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q53EL6

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human protein corresponding to amino acids E346-L446 was used as the immunogen for the PDCD4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the PDCD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PDCD4 antibody is available for research use only.

Storage Conditions

After reconstitution, the PDCD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PDCD4 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with PDCD4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.