PON1 Antibody

Serum paraoxonase/arylesterase 1 (PON1), also known as aromatic esterase 1, is an enzyme that in humans is encoded by the PON1 gene. It is mapped to 7q21.3. This gene has esterase and more specifically paraoxonase activity. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL) . The PON1 gene is activated by PPAR-?, which increases synthesis and release of paraoxonase 1 enzyme from the liver, reducing atherosclerosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, ELISA (Capture; mouse recombinant protein) : 0.1-0.5 µg/mL (BSA-free format available)

UniProt

P52430

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

A recombinant mouse protein corresponding to amino acids A30-D274 was used as the immunogen for the PON1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA (capture)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the PON1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PON1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PON1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PON1 antibody should be determined by the researcher.

Location

Cytoplasmic, secreted

Image Legend

Western blot testing of 1) rat liver, 2) mouse liver, 3) mouse lung and 4) mouse testis tissue lysate with PON1 antibody at 0.5ug/ml. Predicted molecular weight: ~40 kDa.