Leptin Antibody
Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.
Product Specifications
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, ELISA (recombinant rat protein) : 0.1-0.5 µg/mL (BSA-free format available)
UniProt
P50596
Host
Rabbit
Reactivity
Rat
Immunogen
A recombinant rat protein corresponding to amino acids V22-C167 was used as the immunogen for the Leptin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
Limitations
This Leptin antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Leptin antibody should be determined by the researcher.
Image Legend
Western blot testing of recombinant rat protein with Leptin antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.
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