Beta Catenin Antibody

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry: 2-4 µg/mL, Immunofluorescence: 5 µg/mL, FACS: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL (BSA-free formulation available)

UniProt

P35222

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids A2-K233 was used as the immunogen for the Beta Catenin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, ICC, FACS, Direct ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose, 0.025% sodium azide

Reconstitution

After reconstitution, the beta Catenin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Beta Catenin antibody is available for research use only.

Storage Conditions

After reconstitution, the Beta Catenin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Beta Catenin antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Western blot testing of 1) human placenta, 2) human A431, 3) human SK-OV-3, 4) rat heart and 5) mouse testis lysate with Beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.