TIMP4 Antibody

Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. And this gene is mapped to chromosome 3p25. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunofluorescence (FFPE) : 2-4 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested; request BSA-free format for coating)

UniProt

Q99727

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids N174-P224 from the human protein were used as the immunogen for the TIMP4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the TIMP4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TIMP4 antibody is available for research use only.

Storage Conditions

After reconstitution, the TIMP4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TIMP4 antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC testing of FFPE human lung cancer tissue with TIMP4 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.