Bcl-XL Antibody

Bcl-2-like protein 1, also known as Bcl-XL, is a protein that in humans is encoded by the BCL2L1 gene. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

UniProt

Q07817

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids M1-T219 from the human protein were used as the immunogen for the Bcl-XL antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P, IF, IP

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Bcl-XL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Bcl-XL antibody is available for research use only.

Storage Conditions

After reconstitution, the Bcl-XL antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Bcl-XL antibody should be determined by the researcher.

Location

Cytoplasmic, membranous

Image Legend

Flow cytometry testing of fixed and permeabilized human Jurkat cells with Bcl-XL antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Bcl-XL antibody.