Cyclophilin A Antibody / PPIA

Cyclophilin A (PPIA), Peptidylprolyl isomerase A, is an enzyme that in humans is encoded by the PPIA gene. Using chromosome 7 and chromosome 10 deletion hybrid panels, the PPIA coding gene is localized to 7p13-p11.2. This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. Cyclophilin A is also a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and are believed to be involved in protein folding and/or intracellular protein transport. And Cyclophilin A binds to the Gag protein of human immunodeficiency virus type 1 (HIV-1) . Additionally, Cyclophilin A may have an essential function in HIV-1 replication.may have an essential function in HIV-1 replication.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunofluorescence/Immunocytochemistry: 2-4 µg/mL

UniProt

P62937

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids T116-E165 from the human protein were used as the immunogen for the Cyclophilin A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF/ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Cyclophilin A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cyclophilin A antibody is available for research use only.

Storage Conditions

After reconstitution, the Cyclophilin A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Cyclophilin A antibody should be determined by the researcher.

Location

Cytoplasmic, secreted

Image Legend

IHC testing of FFPE human intestinal cancer tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.