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ATP4B Antibody / Gastric hydrogen potassium ATPase beta

This gene encodes the beta subunit of the gastric H+, K+-ATPase. The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H (+) and K (+) ions across the plasma membrane. It is also responsible for gastric acid secretion.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P51164

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 1-28 (MAALQEKKTCGQRMEEFQRYCWNPDTGQ) from the human protein were used as the immunogen for the ATP4B antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the ATP4B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ATP4B antibody is available for research use only.

Storage Conditions

After reconstitution, the ATP4B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ATP4B antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) rat stomach, 2) mouse stomach and 3) human SGC-7901 (human gastric cancer) lysate with ATP4B antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa (core peptide), ~52 kDa (beta subunit precursor), 60-80 kDa (glycosylated form) .

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