TRAIL Antibody

TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253) . It is a member of Tumor Necrosis Factor Ligand Superfamily. In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL has got 281 amino acid proteins. TRAIL mRNA is expressed at significant levels in most normal tissues. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII) and the receptors DcR1 and DcR2, leading to activation of specific kinases and transcription of genes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P50592

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids Y39-N291 from the mouse protein were used as the immunogen for the TRAIL antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the TRAIL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TRAIL antibody is available for research use only.

Storage Conditions

After reconstitution, the TRAIL antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TRAIL antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) mouse Hepa1-6, 2) mouse NIH3T3, 3) rat PC12 and 4) rat NRK cell lysate with TRAIL antibody at 0.5ug/ml. Predicted molecular weight: ~34 kDa (TRAIL), ~20 kDa (soluble TRAIL) .