CD147 Antibody / Basigin

Emmprin, extracellular matrix metalloproteinase inducer, also known as Emmprin (BSG, Basigin) or cluster of differentiation 147 (CD147) is a protein that in humans is encoded by the Emmprin gene. The human gene is mapped to 19p13.3. This protein is a determinant for the Ok blood group system. Basigin has been shown to be an essential receptor on red blood cells for the malaria parasite. It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily, it plays fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development. BSG is thought also to play a role in intercellular recognition. It also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. BSG is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western bot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunofluorescence/Immunocytochemistry (FFPE) : 2-4 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL

UniProt

P35613

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids E138-A323 from the human protein were used as the immunogen for the Basigin / CD147 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/CC, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the Basigin / CD147 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD147 antibody is available for research use only.

Storage Conditions

After reconstitution, the Basigin / CD147 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CD147 antibody should be determined by the researcher.

Location

Membrane

Image Legend

Western blot testing of human 1) HeLa, 2) placenta, 3) Jurkat, 4) U-2 OS, 5) K562, 6) MCF-7, 7) COLO-320, 8) HepG2 and 9) PANC-1 lysate with CD147 antibody. Expected molecular weight: 27-66 kDa depending on level of glycosylation.