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Sca-1 Antibody / Ly6A/E

Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs) . It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P05533

Host

Rabbit

Reactivity

Mouse

Immunogen

Amino acids 48-75 (YPDGVCVTQEAAVIVDSQTRKVKNNLCL) from the mouse protein were used as the immunogen for the Sca-1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the Sca-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Sca-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Sca-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Sca-1 antibody should be determined by the researcher.

Image Legend

Western blot testing of mouse HepA1-6 cell lysate with Sca-1 antibody at 0.5ug/ml. Expected molecular weight: 14-18 kDa.

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