ADAMTS4 Antibody

ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4, is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested; request BSA-free format for coating)

UniProt

O75173

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids 813-837 (TPQDWLHRRAQILEILRRRPWAGRK) from the human protein were used as the immunogen for the ADAMTS4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the ADAMTS4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ADAMTS4 antibody is available for research use only.

Storage Conditions

After reconstitution, the ADAMTS4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ADAMTS4 antibody should be determined by the researcher.

Image Legend

Western blot testing of human recombinant ADAMTS4 partial protein (0.5ng/lane) with ADAMTS4 antibody at 0.5ug/ml. Predicted molecular weight of the full-length protein ~90 kDa.