Nephrin Antibody

NPHS1, also called Nephrin, is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. It gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. A defect in the gene for nephrin, NPHS1, is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine, orproteinuria. NPHS1 is also required for cardiovascular development.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

O60500

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids V1084-V1241 from the human protein were used as the immunogen for the Nephrin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the Nephrin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Nephrin antibody is available for research use only.

Storage Conditions

After reconstitution, the Nephrin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Nephrin antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) rat kidney, 2) mouse kidney and 3) human 293T lysate with Nephrin antibody at 0.5ug/ml. Expected molecular weight: 134 kDa (unmodified), 150-180 kDa (modified) .