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AP2M1 Antibody

AP-2 complex subunit mu is a protein that in humans is encoded by the AP2M1 gene. This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q96CW1

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Amino acids 399-435 (LKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC-human) were used as the immunogen for the AP2M1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the AP2M1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This AP2M1 antibody is available for research use only.

Storage Conditions

After reconstitution, the AP2M1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the AP2M1 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human U-2 OS, 2) human MCF7 and 3) rat kidney lysate with AP2M1 antibody at 0.5ug/ml. Predicted molecular weight ~50 kDa.

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