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EPHX2 Antibody

Soluble epoxide hydrolase (sEH), or Epoxide hydrolase 2, is a bifunctional enzyme that in humans is encoded by the EPHX2 gene. It is mapped to 8p21.2-p21.1. This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells

Gene ID

2053

UniProt

P34913

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 505-543 (QHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLD-human) were used as the immunogen for the EPHX2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the EPHX2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This EPHX2 antibody is available for research use only.

Storage Conditions

After reconstitution, the EPHX2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the EPHX2 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) rat lung, 2) mouse lung and 3) HepG2 lysate with EPHX2 antibody at 0.5ug/ml. Predicted molecular weight: ~63/57 kDa (isoforms 1/2) .

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