Hexokinase II Antibody

Hexokinase II, also known as HK2, is an enzyme which in humans is encoded by the HK2 gene on chromosome 2. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase ii/2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mLWestern blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P52789

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 460-497 (AYRLADQHRARQKTLEHLQLSHDQLLEVKRRMKVEMER-human) were used as the immunogen for the Hexokinase II antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Hexokinase II antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Hexokinase II antibody is available for research use only.

Storage Conditions

After reconstitution, the Hexokinase II antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Hexokinase II antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) U-87 MG, 2) HEL, 3) HepG2 and 4) RT4 cell lysate with Hexokinase II antibody at 0.5ug/ml. Predicted molecular weight ~102 kDa.