Angiogenin Antibody

Angiogenin (ANG1), also known as Ribonuclease 5, is a small 123 amino acid protein that in humans is encoded by the ANG gene. The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL (human protein tested) ; request BSA-free format for coating

Gene ID

283

UniProt

P03950

Host

Rabbit

Reactivity

Human

Immunogen

A human recombinant protein corresponding to amino acids Q25-P147 was used as the immunogen for the Angiogenin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, IHC-P, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Angiogenin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Angiogenin antibody is available for research use only.

Storage Conditions

After reconstitution, the Angiogenin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Angiogenin antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear, secreted

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with Angiogenin antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.