MPS1 Antibody / Metallopan-stimulin 1

40S ribosomal protein S27, also known as Metallopan-stimulin 1 or MPS-1, is a protein that in humans is encoded by the RPS27 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 1-3 µg/mL, Immunocytochemistry: 1-3 µg/mL, Flow cytometry: 1-3ug/10^6 cells

UniProt

P42677

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids P2-H84 from the human protein were used as the immunogen for the MPS1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the MPS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MPS1 antibody is available for research use only.

Storage Conditions

After reconstitution, the MPS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Differences in protocols and secondary/substrate sensitivity may require the MPS1 antibody to be titrated for optimal performance.

Location

Cytoplasmic, membranous

Image Legend

Western blot testing of 1) rat spleen, 2) mouse liver and 3) human HeLa lysate with MPS1 antibody at 0.5ug/ml. Predicted/observed molecular weight ~10 kDa.