RPL19 Antibody / Ribosomal protein L19

60S ribosomal protein L19 is a protein that in humans is encoded by the RPL19 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L19E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence/Immunocytochemistry: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

P84098

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 132-170 (FKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEAR) from the human protein were used as the immunogen for the RPL19 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF/ICC, FACS, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the RPL19 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RPL19 antibody is available for research use only.

Storage Conditions

After reconstitution, the RPL19 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Differences in protocols and secondary/substrate sensitivity may require the RPL19 antibody to be titrated for optimal performance.

Image Legend

IHC staining of FFPE human brain tissue with RPL19 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.