GNB1 Antibody / Guanine nucleotide binding protein beta 1 subunit

Guanine nucleotide-binding protein G (I) /G (S) /G (T) subunit beta-1 is a protein that in humans is encoded by the GNB1 gene. Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IF/ICC (FFPE) : 2-4 µg/mL, FACS: 1-2ug/10^6 cells

UniProt

P62873

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 2-42 (SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR) from the human protein were used as the immunogen for the GNB1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the GNB1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GNB1 antibody is available for research use only.

Storage Conditions

After reconstitution, the GNB1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Differences in protocols and secondary/substrate sensitivity may require the GNB1 antibody to be titrated for optimal performance.

Location

Cytoplasmic, membranous

Image Legend

Western blot testing of 1) rat liver, 2) mouse heart and 3) human HeLa lysate with GNB1 antibody at 0.5ug/ml. Predicted/observed molecular weight: ~37 kDa.