AKR1C1/2 Antibody

AKR1C1 and AKR1C2 are members of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. AKR1C1 binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. The AKR1C1 gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q04828, P52895

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids M1-K123 from human AKR1C1 were used as the immunogen for the AKR1C1/2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the AKR1C1/2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This AKR1C1/2 antibody is available for research use only.

Storage Conditions

After reconstitution, the AKR1C1/2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Differences in protocols and secondary/substrate sensitivity may require the AKR1C1/2 antibody to be titrated for optimal performance.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human HepG2 cells with AKR1C1/2 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.