ADAMTS13 Antibody

ADAMTS13 (A disintegrin and metalloproteinase with athrombospondin type 1 motif, member 13), also known as VWFCP, is a zinc-containing metalloprotease enzyme that cleaves von Willebrand factor (vWf), a large protein involved in blood clotting. It is secreted in blood and degrades large vWf multimers, decreasing their activity. This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. This gene is mapped to 9q34.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, ELISA: 0.5-1 µg/mL (human protein tested) ; request BSA-free format for coating

UniProt

Q76LX8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids A299-R488 from the human protein were used as the immunogen for the ADAMTS13 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA (protein)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the ADAMTS13 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ADAMTS13 antibody is available for research use only.

Storage Conditions

After reconstitution, the ADAMTS13 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Differences in protocols and secondary/substrate sensitivity may require the ADAMTS13 antibody to be titrated for optimal performance.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) rat brain, 2) mouse liver and 3) human HepG2 lysate with ADAMTS13 antibody at 0.5ug/ml. Predicted/observed molecular weight ~154 kDa.