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ABHD5 Antibody / CGI-58

1-acylglycerol-3-phosphate O-acyltransferase ABHD5 is an enzyme that in humans is encoded by the ABHD5 gene. The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated withChanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IF/ICC (FFPE) : 2-4 µg/mL

UniProt

Q8WTS1

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Amino acids R169-D349 from the human protein were used as the immunogen for the ABHD5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the ABHD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ABHD5 antibody is available for research use only.

Storage Conditions

After reconstitution, the ABHD5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Differences in protocols and secondary/substrate sensitivity may require the ABHD5 antibody to be titrated for optimal performance.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) rat kidney and 2) human A431 lysate with ABHD5 antibody at 0.5ug/ml. Predicted molecular weight ~39 kDa.

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