TCP1 beta Antibody / CCT2

T-complex protein 1 subunit beta (TCP1 beta) is a protein that in humans is encoded by the CCT2 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC) . This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P78371

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids E414-C535 from the human protein were used as the immunogen for the TCP1 beta antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

Prior to reconstitution, store at 4oC. After reconstitution, the TCP1 beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TCP1 beta antibody is available for research use only.

Storage Conditions

Prior to reconstitution, store at 4°C. After reconstitution, the TCP1 beta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TCP1 beta antibody should be determined by the researcher.

Location

Cytoplasmic, membranous

Image Legend

Western blot testing of 1) rat brain, 2) rat spleen, 3) rat C6, 4) rat PC-12, 5) mouse brain, 6) mouse spleen, 7) mouse NIH 3T3 and 8) mouse RAW264.7 cell lysate with TCP1 beta antibody. Predicted molecular weight ~57 kDa.