Osteocalcin Antibody

Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. It acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Also, it may play a role in male fertility. And it is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation.

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (FFPE) : 0.5-1 µg/mL

Gene ID

12096

UniProt

P86546

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids Y50-I95 of the mouse protein were used as the immunogen for the Osteocalcin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Osteocalcin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Osteocalcin antibody is available for research use only.

Storage Conditions

After reconstitution, the Osteocalcin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Osteocalcin antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE mouse bone tissue with Osteocalcin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.