Calcium Sensing Receptor Antibody / CASR

The calcium-sensing receptor (CaSR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH) -producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

Gene ID

846

UniProt

P41180

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids Q926-S1078 of the human protein were used as the immunogen for the Calcium Sensing Receptor antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Calcium Sensing Receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Calcium Sensing Receptor antibody is available for research use only.

Storage Conditions

After reconstitution, the Calcium Sensing Receptor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Calcium Sensing Receptor antibody should be determined by the researcher.

Location

Cytoplasmic, membranous

Image Legend

Western blot testing of human 1) HeLa and 2) 22RV1 cell lysate with Calcium Sensing Receptor antibody. Predicted molecular weight ~121 kDa, but is routinely observed at up to ~160 kDa.