VIP Antibody / PHM-27

Vasoactive intestinal peptide, also known as PHM27 or VIP, is a peptide hormone containing 28 amino acid residues. This gene is mapped to 6q25. The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL

Gene ID

7432

UniProt

P01282

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids HADGVFTSDFSKLLGQLSAKKYLESLM (PHM-27 sequence) from the human protein were used as the immunogen for the VIP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the VIP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This VIP antibody is available for research use only.

Storage Conditions

After reconstitution, the VIP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the VIP antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human pancreas with VIP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.