Catalase Antibody

Catalase is a key antioxidant enzyme in the bodies defensing against oxidative stress. It is also a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

Gene ID

847

UniProt

P04040

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids E344-L527 of the human protein were used as the immunogen for the Catalase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Catalase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Catalase antibody is available for research use only.

Storage Conditions

After reconstitution, the Catalase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Catalase antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) rat skeletal muscle, 2) mouse skeletal muscle and 3) human A549 lysate with Catalase antibody. Expected/observed molecular weight ~60 kDa.