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GAL4 Antibody / Galectin-4

Galectin-4 is a protein that in humans is encoded by the LGALS4 gene. This gene is mapped to chromosome 19q13.2 based on an alignment of the LGALS4 sequence. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS4 is an S-type lectin that is strongly underexpressed in colorectal cancer. The 323-amino acid LGALS4 protein contains 2 homologous, approximately 150-amino acid carbohydrate recognition domains and all amino acids typically conserved in galectins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunofluorescence/Immunocytochemistry (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/10^6 cells

Gene ID

3960

UniProt

P56470

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids DRFKVYANGQHLFDFAHRLSAFQRVDTLEIQGDVTLSY were used as the immunogen for the GAL4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF/ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the GAL4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GAL4 antibody is available for research use only.

Storage Conditions

After reconstitution, the GAL4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the GAL4 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) SW620 and 2) COLO320 cell lysate with GAL4 antibody. Expected molecular weight ~36 kDa.

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