TIF1 gamma Antibody / TRIM33

Tripartite motif-containing 33, also known as transcriptional intermediary factor 1 gamma (TIF1-Î³), is a human gene. The TRIM33 gene is mapped to chromosome 1p13 by FISH. The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described; however, the full-length nature of one variant has not been determined.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL

UniProt

Q9UPN9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 1001-1127 of human TIF1 gamma were used as the immunogen for the TIF1 gamma antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the TIF1 gamma antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TIF1 gamma antibody is available for research use only.

Storage Conditions

After reconstitution, the TIF1 gamma antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TIF1 gamma antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human ovarian serous adenocarcinoma tissue with TIF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.