ALIX Antibody / PDCD6IP

Programmed cell death 6-interacting protein, or ALG-2-interacting protein X, is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q8WUM4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 2-330 from the human protein were used as the immunogen for the ALIX antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the ALIX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ALIX antibody is available for research use only.

Storage Conditions

After reconstitution, the ALIX antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ALIX antibody should be determined by the researcher.

Location

Cytoplasm, cell junction, secreted

Image Legend

Western blot testing of 1) rat brain, 2) rat testis, 3) rat liver, 4) mouse liver, 5) mouse spleen, 6) mouse NIH3T3 and 7) human HeLa lysate with ALIX antibody. Expected/observed molecular weight ~96 kDa.