SSH3BP1 Antibody / ABI1

SSH3BP1 is also known as ABI1. ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2. ABI1 participates in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities. It dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins. ABI1 plays a role in the leukemogenesis by translocating to MLL.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunofluorescence: 5 µg/mL

UniProt

Q8IZP0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids HGVKWLKAKHGNNQPARTGTLSRTN of human SSH3BP1 were used as the immunogen for the SSH3BP1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the SSH3BP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SSH3BP1 antibody is available for research use only.

Storage Conditions

After reconstitution, the SSH3BP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SSH3BP1 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Immunofluorescent staining of FFPE human A431 cells with SSH3BP1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.