SKAP55 Antibody / SCAP1 / SKAP1

Src kinase-associated phosphoprotein 1 is an adapter protein that in humans is encoded by the SKAP1 gene. This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

Q86WV1

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Amino acids RMAPHLRRDSKKESCFELTSQDRRSYE of human SKAP55 were used as the immunogen for the SKAP55 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the SKAP55 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SKAP55 antibody is available for research use only.

Storage Conditions

After reconstitution, the SKAP55 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SKAP55 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) PANC and 2) Jurkat cell lysate with SKAP55 antibody. Predicted molecular weight ~41 kDa but routinely observed at ~55 kDa.