SMAD4 Antibody

SMAD4 (Mothers Against Decapentaplegic Drosophila Homolog of 4), also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene. It belongs to the Darfwin family of proteins that modulate members of the TGFb protein superfamily. Hahn et al. (1996) identified the SMAD4 gene on chromosome 18q21.1. Howe et al. (1998) identified the SMAD4 gene within a region on 18q21.1 defined by linkage analysis in kindred with juvenile polyposis syndrome. To test directly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands. SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL

UniProt

Q13485

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids EIHLHRALQLLDEVLHTMPIADPQPLD of human SMAD4 were used as the immunogen for the SMAD4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the SMAD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SMAD4 antibody is available for research use only.

Storage Conditions

After reconstitution, the SMAD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SMAD4 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) rat brain, 2) mouse brain, 3) rat skeletal muscle, 4) mouse skeletal muscle, human 5) U87, 6) placenta, 7) HT1080, 8) HeLa and 9) NEURO lysate with SMAD4 antibody. Expected/observed molecular weight ~60 kDa.