BIK Antibody / BCL2-interacting killer

Bcl-2-interacting killer is a protein that in humans is encoded by the BIK gene. This gene is mapped to 22q13.31. The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells

UniProt

Q13323

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 1-123 of human BIK were used as the immunogen for the BIK antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the BIK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This BIK antibody is available for research use only.

Storage Conditions

After reconstitution, the BIK antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the BIK antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) rat spleen, 2) rat stomach, 3) rat intestine, 4) human MCF7, 5) human A549 and 6) human SKOV lysate with BIK antibody. Expected molecular weight ~18 kDa but routinely observed at 18-22 kDa.